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13-09-2019
Biology

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During purification of many cytosolic proteins, it is important to
include either dithiothreitol or b-mercaptoethanol in the
buffers. Why?

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palipali964 palipali964

21-09-2019

Answer:

To reduce thiol-groups of the protein

Explanation:

Dithiothreitol of b-mercaptoethanol act as reducing agents. Including them in a buffer would prevent oxidation of thiol-groups in amino acids of the protein and preventing its denaturation.

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